mouse r d systems bba3 il 1a  (R&D Systems)

Journal: Journal of oral biosciences

Article Title: P2 purinergic receptor signaling and interleukin-1 synergistically induce interleukin-6 production in a human oral squamous carcinoma cell line.

doi: 10.1016/j.job.2021.01.004

Figure Lengend Snippet: Fig. 6. Interleukin (IL)-6 production by Ca9-22 and HO-1-u-1 cells stimulated with adenine nucleotides and IL-1. (A and C) The mRNA expression levels of IL-1R1, IL-1RAP, IL-1a and IL-1b in Ca9-22 (A) and HO-1-u-1 (C) cells were analyzed using reverse transcription polymerase chain reaction (RT-PCR). (B and D) Ca9-22 (B) or HO-1-u-1 (D) cells were stimulated with 100 mM adenine nucleotides and 10 ng/ml IL-1a or b for 24 h. The concentrations of IL-6 in culture supernatants were measured using enzyme-linked immu- nosorbent assay (ELISA). **P < 0.01, significantly different from the control (without IL-1). Values in the graphs are expressed as the mean ± standard deviation (SD) of a single experiment performed in triplicate.

Article Snippet: UDP, Evans blue, pyridoxalphosphate-6-azophenyl-20,40-disulfonic acid (PPADS), suramin, and anti-human IL-1a antibody were purchased from Abcam (Cambridge, UK).

Techniques: Expressing, Reverse Transcription, Polymerase Chain Reaction, Reverse Transcription Polymerase Chain Reaction, Enzyme-linked Immunosorbent Assay, Control, Standard Deviation

Journal: Journal of oral biosciences

Article Title: P2 purinergic receptor signaling and interleukin-1 synergistically induce interleukin-6 production in a human oral squamous carcinoma cell line.

doi: 10.1016/j.job.2021.01.004

Figure Lengend Snippet: Fig. 7. Effects of mitogen-activated protein kinase (MAPK) inhibitors on interleukin (IL)-6 production by HSC-2 cells stimulated with adenine nucleotides and IL-1a. (AeC) HSC-2 cells were stimulated with 100 mM ATP or ADP and 10 ng/ml IL-1a for 10 min. Whole cell lysates (2.5 mg protein in each lane) were analyzed using western blotting with anti- p38 and anti-p-p38 (A), anti-JNK and anti-p-JNK (B), and anti-ERK and anti-p-ERK (C) antibodies. Data shown are representative of three independent experiments. Bars show the mean ± standard deviation (SD) of three or four independent experiments. (DeF) Cells were preincubated for 30 min with the p38 inhibitor SB203580 (D), the JNK inhibitor SP600125 (E), or the ERK inhibitor PD98059 (F) at the indicated concentrations, then further stimulated with 100 mM ATP or ADP and 10 ng/ml IL-1a for 24 h. The concentrations of IL-6 in culture supernatants were measured using enzyme-linked immunosorbent assay (ELISA). **P < 0.01, *P < 0.05, significantly different from the control (0 mM inhibitor). Values in the graphs are expressed as the mean ± SD of a single experiment performed in triplicate.

Article Snippet: UDP, Evans blue, pyridoxalphosphate-6-azophenyl-20,40-disulfonic acid (PPADS), suramin, and anti-human IL-1a antibody were purchased from Abcam (Cambridge, UK).

Techniques: Western Blot, Standard Deviation, Enzyme-linked Immunosorbent Assay, Control